byLippow, Shaun M.; Aha, Patti M.; Parker, Matthew H.; Blake, William J.; Baynes, Brian M.; Lipovsek, Dasa
This article is from Nucleic Acids Research, volume 37.AbstractType IIS restriction endonucleases cleave DNA outside their recognition sequences, and are therefore particularly useful in the assembly of DNA from smaller fragments. A limitation of type IIS restriction endonucleases in assembly of long DNA sequences is the relative abundance of their target sites. To facilitate ligation-based assembly of extremely long pieces of DNA, we have engineered a new type IIS restriction endonuclease that... Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2685105
byBlake, William J.; Chapman, Brad A.; Zindal, Anuradha; Lee, Michael E.; Lippow, Shaun M.; Baynes, Brian M.
This article is from Nucleic Acids Research, volume 38.AbstractThe engineering of biological components has been facilitated by de novo synthesis of gene-length DNA. Biological engineering at the level of pathways and genomes, however, requires a scalable and cost-effective assembly of DNA molecules that are longer than ∼10 kb, and this remains a challenge. Here we present the development of pairwise selection assembly (PSA), a process that involves hierarchical construction of long-length... Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2860126